ABSTRACT
Aims: To evaluate utility of replication system using chromogenic disc for detection of uropathogens and to compare the result with conventional method for the same. Design: A total of 625 urine samples were processed from suspected cases of urinary tract infection, admitted in a rural medical college of Maharashtra. Methodology: The culture isolates of uropathogens were identified by both conventional method and by chromogenic disc using replica system. Results: Out of 625 urine specimens, 419 (67.04%) were Culture positive. There was growth of Escherichia coli 197(99.49%), Enterococcus faecalis 88 (21%), Klebsiella pneumoniae 63 (15.03%) and Pseudomonas aeruginosa 10.73%. There was mixed growth of organisms 15(3.57%) in urine specimens. All uropathogens isolates were identified correctly by conventional as well as chromogenic disc using replica system, except one. One of the Escherichia coli isolate was identified by conventional methods but with replica system it showed colourless colonies instead of purple colonies. Conclusion: Replica system is a rapid, cost effective and easy method for detection of uropathogens with satisfactory result. It can be adopted in clinical microbiology laboratory for presumptive diagnosis of uropathogens.
ABSTRACT
Background and objectives: Acinetobacter baumannii is emerging as an important pathogen causing hospital acquired infections. The present study was directed to find out the incidence, antibiotic susceptibility and metallo β lactamases production of Acinetobacter baumannii isolated from various clinical samples, in an intensive care unit. Methods: Isolation of Acinetobacter baumannii from various clinical samples was done. The isolates were tested for antibiotic sensitivity as per conventional methods. Imipenem resistant isolates were further tested for MBL production by double disk synergy test and MBL E test. Results: Total number of Acinetobacter baumannii isolates from clinical samples was 48. Maximum number of isolates were from blood (31.25%) followed by endotracheal aspiration (25%). Total 10 (20.83%) isolates were imipenem resistant, among which, 9(18.75%) were metallo β lactamases producers. MBL producers were more resistant to commonly used antibiotics than its non MBL producing counter parts. All isolates were susceptible to colistin (10 μg), polymyxin B (300 μg) and tigecycline (15μg).Conclusions: Multidrug resistant, metallo β lactamases producing Acinetobacter baumannii infection was not uncommon in our intensive care unit. Colistin, polymyxin B and tigecycline were very effective against such isolates.